Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Neutrophil Extracellular Traps Mediate Acute Liver Failure in Regulation of miR-223/Neutrophil Elastase Signaling in Mice

doi: 10.1016/j.jcmgh.2022.05.012

Figure Lengend Snippet: Sivelestat-mediated NE blockade protects against ALF in mice. ( A ) Ten-week-old male C57 BL/6J mice were pretreated with sivelestat (SIV, 150 mg kg -1 body weight) via a single intraperitoneal injection 24 hours before ALF induction with intraperitoneal injection of D-GalN (0.75 mg g -1 body weight) and LPS (2.5 μg g -1 body weight). ( B ) Histologic lesions in the liver were assessed by H&E staining as shown in representative images (original magnification, 400×). Scale bar : 10 μm. ( C ) Quantification of necrotic area in H&E-stained liver sections. Serum activity of ( D ) ALT and ( E ) AST was measured to evaluate hepatocellular damage. ( F ) mRNA expression level of TNF-α in the liver tissue determined by quantitative PCR. ( G ) Representative images of immunohistochemistry of MPO in liver section ( upper ; scale bar: 10 μm ) and semiquantification of MPO + cells in each 40× microscopic field (lower). ( H ) Immunoblotting analysis of MPO in liver lysate ( upper ) and densitometric analysis of MPO protein abundance relative to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as loading control ( lower ). Data are expressed as means ± SEM. n = 5–6. ∗ P < .05, ∗∗ P < .01.

Article Snippet: Ten-week-old male C57BL/6J mice received a peritoneal injection of either Sivelestat (pharmacologic inhibitor against NE, 150 mg kg -1 body weight, dissolved in 5% dimethyl sulfoxide, 40% polyethylene glycol 300, 5% Tween 80, 50% double-distilled H 2 O, 127373-66-4; Selleck, Houston, TX) or GSK484 (pharmacologic inhibitor against NETs, 20 mg kg -1 body weight, dissolved in PBS, SML1658; Sigma-Aldrich) 24 hours before ALF induction.

Techniques: Injection, Staining, Activity Assay, Expressing, Real-time Polymerase Chain Reaction, Immunohistochemistry, Western Blot, Quantitative Proteomics, Control

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Neutrophil Extracellular Traps Mediate Acute Liver Failure in Regulation of miR-223/Neutrophil Elastase Signaling in Mice

doi: 10.1016/j.jcmgh.2022.05.012

Figure Lengend Snippet: Pharmacologic inhibition of NE protects against ALF in miR-223 KO mice and WT control mice. ( A ) The pretreatment with sivelestat (SIV) was conducted in 10-week-old male miR-223 KO mice and WT control mice via a single intraperitoneal injection of D-GalN (0.75 mg g -1 body weight) and LPS (2.5 μg g -1 body weight) 24 hours before ALF induction. ( B ) Histologic lesions were assessed by H&E staining in liver sections as shown in representative images (original magnification, 400×) Scale bar : 10 μm. ( C ) Quantification data of necrotic area in H&E-stained liver sections. Serum levels of ( D ) ALT and ( E ) AST was measured to evaluate hepatocellular damage. ( F ) TNF-α mRNA levels in liver tissue were determined by quantitative PCR. Data are expressed as means ± SEM. n = 5–6. ∗ P < .05, ∗∗ P < .01.

Article Snippet: Ten-week-old male C57BL/6J mice received a peritoneal injection of either Sivelestat (pharmacologic inhibitor against NE, 150 mg kg -1 body weight, dissolved in 5% dimethyl sulfoxide, 40% polyethylene glycol 300, 5% Tween 80, 50% double-distilled H 2 O, 127373-66-4; Selleck, Houston, TX) or GSK484 (pharmacologic inhibitor against NETs, 20 mg kg -1 body weight, dissolved in PBS, SML1658; Sigma-Aldrich) 24 hours before ALF induction.

Techniques: Inhibition, Control, Injection, Staining, Real-time Polymerase Chain Reaction

Journal: AMB Express

Article Title: Influence of culture media, pH and temperature on growth and bacteriocin production of bacteriocinogenic lactic acid bacteria

doi: 10.1186/s13568-018-0536-0

Figure Lengend Snippet: Growth curves generated from the Bioscreen C for Lactobacillus curvatus (Arla-10), Enterococcus faecium (JFR-1), Lactobacillus paracasei subsp. paracasei (JFR-5), and Streptococcus thermophilus (TSB-8) at their optimal growth conditions for 48 h. Arla-10 strain was cultured in MRS broth, initial pH 7.4, at 37 °C ( a ); JFR-1 strain was cultured in BHI broth, initial pH 7.4, at 37 °C ( b ); JFR-5 strain was cultured in BHI broth, initial pH 7.4, at 37 °C ( c ); TSB-8 strain was cultured in BHI broth, initial pH 8.5, at 37 °C ( d ). *The OD value was measured and recorded by the Bioscreen C at 20-min interval. The numbers of 20, 40, 60, 80 100, 120 and 140 on the X axis represent that LAB has grown for 400, 800, 1200, 1600, 2000, 2400 and 2800 min, respectively

Article Snippet: Arla-10, JFR-1 and JFR-5 strains were cultured in MRS broth (10 g peptone, 8.0 g lab-lemco’ powder, 4.0 g yeast extract, 20 g glucose, 1 ml Tween’ 80, 2.0 g di-potassium hydrogen phosphate, 5.0 g sodium acetate 3 H 2 O, 2.0 g tri-ammonium citrate, 0.2 g magnesium sulphate 7 H 2 O, and 0.05 g manganese sulphate 4 H 2 O per liter of water, pH 6.2 ± 0.2) (Oxoid, Basingstoke, UK).

Techniques: Generated, Cell Culture

Journal: AMB Express

Article Title: Influence of culture media, pH and temperature on growth and bacteriocin production of bacteriocinogenic lactic acid bacteria

doi: 10.1186/s13568-018-0536-0

Figure Lengend Snippet: OD values, viability, and antimicrobial activities (AU) of CFS and BLS of Arla-10 strain at different growth conditions. (●) MRS, pH 6.2, 37 °C; (○) MRS, pH 6.2, 44 °C; (■) MRS, pH 7.4, 37 °C; (□) MRS, pH 7.4, 44 °C; (▲) BHI, pH 5.5, 37 °C; (△) BHI, pH 5.5, 44 °C; (▼) BHI, pH 6.2, 37 °C; (▽) BHI, pH 6.2, 44 °C. a Optical density (OD); b LAB viable counts; c antimicrobial activity of CFS and d antimicrobial activity of BLS. The experimental data were fitted to the logistic model. The vertical bar represents standard errors of the means

Article Snippet: Arla-10, JFR-1 and JFR-5 strains were cultured in MRS broth (10 g peptone, 8.0 g lab-lemco’ powder, 4.0 g yeast extract, 20 g glucose, 1 ml Tween’ 80, 2.0 g di-potassium hydrogen phosphate, 5.0 g sodium acetate 3 H 2 O, 2.0 g tri-ammonium citrate, 0.2 g magnesium sulphate 7 H 2 O, and 0.05 g manganese sulphate 4 H 2 O per liter of water, pH 6.2 ± 0.2) (Oxoid, Basingstoke, UK).

Techniques: Activity Assay

Journal: AMB Express

Article Title: Influence of culture media, pH and temperature on growth and bacteriocin production of bacteriocinogenic lactic acid bacteria

doi: 10.1186/s13568-018-0536-0

Figure Lengend Snippet: OD values, viability, and antimicrobial activities (AU) of CFS and BLS of JFR-1 strain at different growth conditions. (●) MRS, pH 6.2, 37 °C; (○) MRS, pH 6.2, 44 °C; (■) MRS, pH 7.4, 37 °C; (□) MRS, pH 7.4, 44 °C; (▲) BHI, pH 5.5, 37 °C; (△) BHI, pH 5.5, 44 °C; (▼) BHI, pH 6.2, 37 °C; (▽) BHI, pH 6.2, 44 °C. a Optical density (OD); b LAB viable counts; c antimicrobial activity of CFS and d antimicrobial activity of BLS. The experimental data were fitted to the logistic model. The vertical bar represents standard errors of the means

Article Snippet: Arla-10, JFR-1 and JFR-5 strains were cultured in MRS broth (10 g peptone, 8.0 g lab-lemco’ powder, 4.0 g yeast extract, 20 g glucose, 1 ml Tween’ 80, 2.0 g di-potassium hydrogen phosphate, 5.0 g sodium acetate 3 H 2 O, 2.0 g tri-ammonium citrate, 0.2 g magnesium sulphate 7 H 2 O, and 0.05 g manganese sulphate 4 H 2 O per liter of water, pH 6.2 ± 0.2) (Oxoid, Basingstoke, UK).

Techniques: Activity Assay

Journal: AMB Express

Article Title: Influence of culture media, pH and temperature on growth and bacteriocin production of bacteriocinogenic lactic acid bacteria

doi: 10.1186/s13568-018-0536-0

Figure Lengend Snippet: OD values, viability, and antimicrobial activities (AU) of CFS and BLS of JFR-5 strain under different growth conditions. (●) MRS, pH 6.2, 37 °C; (○) MRS, pH 6.2, 44 °C; (■) MRS, pH 7.4, 37 °C; (□) MRS, pH 7.4, 44 °C; (▲) BHI, pH 5.5, 37 °C; (△) BHI, pH 5.5, 44 °C; (▼) BHI, pH 6.2, 37 °C; (▽) BHI, pH 6.2, 44 °C. a Optical density (OD); b LAB viable counts; c antimicrobial activity of CFS and d antimicrobial activity of BLS. The experimental data were fitted to the logistic model. The vertical bar represents standard errors of the means

Article Snippet: Arla-10, JFR-1 and JFR-5 strains were cultured in MRS broth (10 g peptone, 8.0 g lab-lemco’ powder, 4.0 g yeast extract, 20 g glucose, 1 ml Tween’ 80, 2.0 g di-potassium hydrogen phosphate, 5.0 g sodium acetate 3 H 2 O, 2.0 g tri-ammonium citrate, 0.2 g magnesium sulphate 7 H 2 O, and 0.05 g manganese sulphate 4 H 2 O per liter of water, pH 6.2 ± 0.2) (Oxoid, Basingstoke, UK).

Techniques: Activity Assay

Journal: AMB Express

Article Title: Influence of culture media, pH and temperature on growth and bacteriocin production of bacteriocinogenic lactic acid bacteria

doi: 10.1186/s13568-018-0536-0

Figure Lengend Snippet: pH changes of Lactobacillus curvatus (Arla-10) ( a ), Enterococcus faecium (JFR-1) ( b ), Lactobacillus paracasei subsp. paracasei (JFR-5) ( c ), and Streptococcus thermophilus (TSB-8) ( d ) grown under different growth conditions. (●) MRS, pH 6.2, 37 °C; (○) MRS, pH 6.2, 44 °C; (■) MRS, pH 7.4, 37 °C; (□) MRS, pH 7.4, 44 °C; (▲) BHI, pH 5.5, 37 °C; (Δ) BHI, pH 5.5, 44 °C; (▼) BHI, pH 6.2, 37 °C; (▽) BHI, pH 6.2, 44 °C. The experimental data were fitted to the logistic model. The vertical bar represents standard errors of the means

Article Snippet: Arla-10, JFR-1 and JFR-5 strains were cultured in MRS broth (10 g peptone, 8.0 g lab-lemco’ powder, 4.0 g yeast extract, 20 g glucose, 1 ml Tween’ 80, 2.0 g di-potassium hydrogen phosphate, 5.0 g sodium acetate 3 H 2 O, 2.0 g tri-ammonium citrate, 0.2 g magnesium sulphate 7 H 2 O, and 0.05 g manganese sulphate 4 H 2 O per liter of water, pH 6.2 ± 0.2) (Oxoid, Basingstoke, UK).

Techniques: